Orc2 antibody drosophila

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August undefined, 2024

WebOct 9, 2024 · Meier-Gorlin syndrome (MGS) is a rare autosomal recessive disorder characterized by microtia, primordial dwarfism, small ears and skeletal abnormalities. … WebThe newly identified 30-kDa protein hsORC6 is 28% identical and 49% similar to ORC6p from Drosophila melanogaster, which is consistent with the identities and similarities found …

Humanized Drosophila Model of the Meier-Gorlin Syndrome …

WebThe origin-recognition complex (ORC) has an essential role in defining DNA replication origins [1] and in chromosome segregation [2, 3]. Recent studies in Drosophila orc2 mutants [4–6], and in human cells depleted of ORC2 [3], have suggested that this factor is also implicated in mitotic chromosome assembly. We asked whether ORC was required for M … WebPhosphospecific antibodies were generated using synthetic peptides corresponding to amino acids 112–120 (CELAKpTPQKS) and 221–230 (CPVGKEpTPSKR) of ORC2 for anti … devkamal hospital \u0026 research centre

ORC localization in Drosophila follicle cells and the effects of ...

WebApr 13, 2024 · This study shows that Drosophila germ granules, which initially protect RNAs, subsequently become sites of selective mRNA degradation; this plasticity supports germ cell development. ... 50 μg/mL tRNA), embryos were incubated with primary antibody in PBHT overnight at 4°C with rocking. Primary antibodies used: 1:250 rabbit anti-CCR4 , 1:250 ... WebAug 30, 2011 · ORC function is required to allow both stages of replication initiation at DAFC-34B. DNA copy number was examined in a female-sterile allele of ORC, orc2fs293, which reduces gene amplification. orc2k43 is a null allele. Amplification in the sibling heterozygotes shows typical amplification levels. WebGene: orc-2 Immunogen: Recombinant His6-tagged ORC-2 fusion protein Alternate Gene Names: Alternate Antibody Name: 4D4 Clonality: Monoclonal Alternate Antigen Name: … dev karma-webpack 3.0.0 from the root project

Functional analysis of mutant and wild-type Drosophila …

WebDec 14, 2000 · This pattern of staining was also observed using affinity-purified ORC2 antibody and in three Drosophila cell lines following either paraformaldehyde or methanol fixation (Fig. 2 b). ORC2 staining is remarkably similar to that of Xenopus ORC1, which is also absent from metaphase chromosomes but present on anaphase chromatids [33]. It … WebPreparation of the ORC2 antibodies. The Drosophila ORC2 coding sequence was PCR amplified from a 4- to 8-hr embryonic cDNA library (Brown and Kafatos 1988). The PCR product was subcloned into pCYB2 (New England Biolabs) usingNdeI and SmaI sites engineered into the termini of the PCR product. DmORC2 protein was expressed in … churchill hellWebOct 2, 2001 · Immunostaining of the Drosophila embryos was performed as described by using affinity-purified antibody raised against Drosophila ORC2 and ORC6 proteins. Results Six different mutant complexes and wild-type recombinant ORC were prepared as … churchill helado

"WebThe origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast … " - Orc2 antibody drosophila

Orc2 antibody drosophila

Role for a Drosophila Myb-containing protein complex in …

WebJun 24, 2004 · The centrosomes of HeLa cells arrested in the aberrant mitotic state failed to label with anti-Orc2 antibodies, suggesting that the Orc2 centrosome labeling was specific (Supplementary Figure S10B). ... Mutations in Drosophila Orc2 also disrupt HP1 association with heterochromatin (Huang et al, 1998). WebApr 1, 1999 · We isolated mutations in Drosophila E2F and DP that affect chorion gene amplification and ORC2 localization in the follicle cells. In the follicle cells of the ovary, the ORC2 protein is localized throughout the follicle cell nuclei when they are undergoing polyploid genomic replication, and its levels appear constant in both S and G phases.

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WebNov 2, 2011 · We thank I. Chesnokov and the Bloomington Drosophila Stock Center (Bloomington, IN) for flies, S. Bell and M. Botchan for α-Orc2 antibodies, J. Powers of the Indiana Light Microscopy Imaging Center (Bloomington, IN) for helpful advice, and M. Lilly and members of the Calvi lab for advice and comments on the manuscript. We thank M. … WebNational Center for Biotechnology Information

WebJul 30, 2008 · We showed previously by coimmunoprecipitation that Drosophila Orc1 and Orc2 proteins interact with the dDP/dE2F1/Rbf1 complex in ovarian extracts, and we wished to further characterize this interaction . ... Anti-HA (Sigma), Anti-ORC2, and anti-dE2F1 antibodies were used at 1∶100 dilutions. Anti- RBF2 was used at 1∶1 dilutions. Anti-V5 ...

WebOct 8, 2010 · IP with Orc2 antibody followed by immunoblot with ORCA polyclonal antibody (pAb) showed the association of Orc2 with endogenous ORCA (molecular weight of ORCA ∼70kDa; Figure 1 D). Finally, immunoprecipitation with ORCA pAb in human cells clearly demonstrated the association of ORCA with ORC subunits ( Figure 1 E). WebMay 1, 2004 · Antibody to the origin recognition complex subunit 2 (ORC2)recognizes large foci that localize to the endogenous chorion gene loci and to active transgenic constructs …

WebDrosophila Myb recognizes a highly conserved DNA sequence10,11, but the speciﬁc binding properties of the glycerol gradient fractions might be more complex than that of …

WebFeb 15, 2024 · Gene Summary Origin recognition complex subunit 2 ( Orc2) encodes a subunit of the origin recognition complex (ORC), which is essential for the initiation of … churchill hellraisersWebAbout 20% of the Orc2 subunit is eluted from the nuclei after an RNase treatment ... anti-rabbit IgG (Amersham) and Alexa Fluor 488-conjugated goat anti-mouse IgG (Molecular Probes) were used as secondary antibodies. Drosophila cell culture extracts. Drosophila Schneider line 2 (S2) cells were maintained at 25°C in Schneider's Insect Medium ... churchill help supportWebJan 12, 2007 · We find that the developmental timing, epigenetic regulation, and Orc2 protein binding have been strictly conserved over 40 million years of evolution. We also present evidence that additional loci may be amplified in some species. devkaransingh56 gmail.comWebDec 1, 2024 · To analyze the ability of mutant proteins to interact with the rest of the complex, we immunoprecipitated ORC from obtained transgenic flies using Orc2 … churchill heirs europeWebDrosophila provides a powerful model for understanding replication control in metazoans. ... The anti-ORC2 antibodies were provided by Richard Austin and Steve Bell and used at a dilution of 1:2500. Anti-DUP antibodies were used at a dilution of 1:1000. Microscopy for fluorescently labeled embryos and ovaries was done using either a Biorad ... churchill heritage centreWebDec 1, 2024 · Protein A-Sepharose (#6501-5; BioVision) and rabbit polyclonal antibodies against Drosophila Orc2 subunit were added and incubated with the supernatant overnight. Sepharose beads were washed three times with low-salt immunoprecipitation buffer and diluted in 10 µl of immunoprecipitation buffer. churchill hell quoteWebContact Cusabio to Customize Orc2 Antibody, which reacts with Drosophila melanogaster (Fruit fly) and will validated for ELISA, WB (ensure identification of antigen). Call: 301-363-4651; ... Rabbit anti-Drosophila melanogaster (Fruit fly) Orc2 Polyclonal antibody: Uniprot No. Q24168: Target Names: Orc2: Alternative Names: devk benefits corporate