Flp-intm t-rextm 293 细胞系
Web293t细胞由293细胞通过基因技术派生出的细胞系,是经过腺病毒e1a基因的转染,能表达sv40大t抗原,含有sv40复制起始点与启动子区。 许多真核表达载体如pcDNA3.1中含有SV40病毒的复制起始位点,可以在表达SV40病毒T抗原的细胞系中复制,从而提高外源基因 … http://www.bio-thing.com/p118025
Flp-intm t-rextm 293 细胞系
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WebFigure 5-9. PMA induces internalisation of hFFA4. Flp-InTM T-RExTM 293 cells able to express FLAG-hFFA4-mVenus were plated down onto poly-D-lysine-coated 30 mm glass coverslips and receptor expression was induced using doxycycline (100 ng/ml). The internalisation of hFFA4 in live cells was then imaged using a Zeiss VivaTome spinning … WebFlp-In TM T-REx TM 系统将Flp-In TM 系统的靶向整合与T-REx TM 系统的强大诱导表达能力整合在一起。该系统能够生成同基因,可诱导的稳定表达细胞系,并能够针对这些细 …
WebFeb 13, 2024 · flp/frt 系统就属于位点特异性重组系统的一种。 FLP是一个重组酶(flippase,Flp),类似于Cre,它识别的位点是FRT,当FRT在同一条DNA上且方向相 … WebThe T-REx™ Cell Lines are functionally tested by transient transfection with the positive control vector pcDNA™4⁄TO⁄lacZ. T-REx™ Cell Lines exhibit extremely low basal …
WebJul 2, 2012 · Als Kontrollen dienten mit dem Leervektor transfizierte und hCtr1 überexprimierende Flp-InTM T-RExTM-293-Zellen. Die rekombinanten hCtr2-Zellen erwiesen sich als signifikant weniger sensitiv gegenüber Cis- und Carboplatin als die Kontrollzellen. hCtr2 hatte hier also einen resistenzfördernden Effekt. Dies ist ein … WebFlp-InTM T-RExTM 293 cells stably harboring hFFA2-eYFP at the Flp-InTM T-RExTM locus were induced to express the receptor. The capacity of GLPG0974 to inhibit elevation of [Ca2+] i. produced by EC80 concentrations of C3 or Cmp 1 is shown (B). Membranes from such induced cells were used to assess the ability of GLPG0974 to inhibit the binding of ...
WebAbstract. Each subtype of the muscarinic receptor family of G protein-coupled receptors is activated by similar concentrations of the neurotransmitter acetylcholine or closely rel
WebFlp-In ™ 293 T-REx 细胞系设计用于快速生成稳定细胞系,确保通过 Flp-In™ 表达载体对您的目标蛋白质进行均一表达。这些细胞在转录活性基因组基因座处包含一个稳定整合的 … inchcape melbourneWebFlp-In™ stable cell lines are isogenic and hence protein expression is constant across a population of cells. A useful addition to the Flp-In™ system (Flp-In™ T-Rex™) allows … inchcape mckayWebOct 25, 2024 · The Flp-InTM T-RExTM 293 cells were generated by cotransfection of the constructed plasmid and pOG44 plasmid to express the target gene. Successfully recombined monoclonal cell lines were screened ... inchcape management services limitedWebFeb 20, 2024 · 在我们以前的工作中,我们在四环素诱导型CMV启动子(HEK-293 Flp-In T-REx-Thermo)的控制下,建立了表达C末端3xFLAG标记的外来体成分EXOSC10(RRP6)的同基因HEK-293细胞系费雪科学)。. 该系统允许我们以与内源WT蛋白质相当的水平表达标记的EXOSC10蛋白质,并且使用磁性 ... inchcape mckay shipping ltdWebSep 29, 2024 · 293FTM 细胞 是由 Flp-InTM T-RExTM 293 ... 总之, 293系列细胞经过长时间的改造与优化,已在多个领域实现了较多的应用,源井生物对于293与293T细胞也有丰富的基因编辑和细胞模型构建的经验,并拥有上千种293细胞的KO现货,覆盖超20种信号通 … income tax short form 1040aWebThe Flp-InTM T-RExTM 293 cells were generated by cotransfection of the constructed plasmid and pOG44 plasmid to express the target gene. Successfully recombined monoclonal cell lines were screened by hygromycin B resistance. Western blot and indirect immunofluorescence (IFA) were used to examine the expression of the target protein in ... income tax sharesWebATCC flp intm t rextm 293 cells Flp Intm T Rextm 293 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS … inchcape malaysia